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antibodies against agrin  (R&D Systems)


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    Structured Review

    R&D Systems antibodies against agrin
    Antibodies Against Agrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+agrin/Agrin+Antibody+(AGR+131)/pmc07003618-39-23-26
    Average 90 stars, based on 1 article reviews
    antibodies against agrin - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto Hybond-P polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween-20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4°C overnight. .. After incubating with IRDye800-conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling.
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto HybondP polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4◦C overnight. .. After incubating with IRDye800conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).

    Saline:

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto Hybond-P polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween-20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4°C overnight. .. After incubating with IRDye800-conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling.
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto HybondP polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4◦C overnight. .. After incubating with IRDye800conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).

    Incubation:

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto Hybond-P polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween-20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4°C overnight. .. After incubating with IRDye800-conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).

    Article Title: Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling.
    Article Snippet: Protein samples (20 μg/lane) were separated by SDS-PAGE and transferred onto HybondP polyvinylidene difluoride (PVDF) membranes (Millipore, United States). .. After blocking in 5% (w/v) BSA (Sigma, United States) and washing with tris-buffered saline with Tween20 (TBST), the membranes were then incubated with antibodies against agrin (R&D Systems, Minnesota, CA, United States), anti-MuSK (Abcam, United States), and anti-GAPDH (Abcam, United States) at 4◦C overnight. .. After incubating with IRDye800conjugated secondary antibody (Rockland, Philadelphia, PA, United States) for 1 h at room temperature and washing with TBST, images were acquired and band density was analyzed using Odyssey Infrared Imaging System (LI-COR Biosciences, United States).



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    Image Search Results


    List of seven high-potential candidate proteins for PTC biomarkers

    Journal: Oncotarget

    Article Title: Integrated analysis of fine-needle-aspiration cystic fluid proteome, cancer cell secretome, and public transcriptome datasets for papillary thyroid cancer biomarker discovery

    doi: 10.18632/oncotarget.23951

    Figure Lengend Snippet: List of seven high-potential candidate proteins for PTC biomarkers

    Article Snippet: Cell extracts and conditioned media (50 μg protein) were resolved by SDS-PAGE, transferred to PVDF membranes (pore size, 0.45 μm; Millipore), and probed with antibodies against agrin (AGRN) (1:1000 dilution; sc-6166, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CD26/DPP4 (1:1000 dilution; BAF1180, R&D Systems, Minneapolis, MN, USA), NPC2 (1:1000 dilution, sc-166321, Santa Cruz Biotechnology), GPNMB (1:500 dilution; BAF2550, R&D Systems), galectin-3 (LGALS3; 1:1000 dilution; MAB4033, Chemicon International, Temecula, CA, USA), ERAP2 (1:1000 dilution; AF3830, R&D Systems), CTSC (1:1000 dilution, sc-74590, Santa Cruz Biotechnology), SFTPB (PSPB; 1:1000 dilution, sc-133143, Santa Cruz Biotechnology), tubulin (1:3000 dilution; sc-8035, Santa Cruz Biotechnology), and actin (1:5000 dilution; MAB1501, Millipore).

    Techniques: Membrane